Evolution of Enzyme Function

Using directed and experimental evolution we adapt micro-organisms in the laboratory to novel environmental conditions. Using genomics and biochemistry we study how adaptive mutations fixate and decipher the molecular mechanism involved in survival. Particularly, we are interested in understanding the selection pressures that shape an enzyme’s optimal efficiency and the role of promiscuous enzymes in mediating adaptation to novel environments and stressful conditions.

Engineering Bioremediation Tools

Combining semi-rational protein and metabolic engineering we are developing synthetic cells for the depolymerization and assimilation of recalcitrant plastic polymers and organophosphates.