Evolution of enzyme functions in biofilms

Using directed and experimental evolution we adapt biofilms in the laboratory to novel environmental conditions. Using genomics and biochemistry we study how adaptive mutations fixate and decipher the molecular mechanism involved in biofilm survival. Particularly, we are interested in understanding the selection pressures that shape an enzyme’s optimal k   and k     in biofilms.



Biofilms underground metabolism

Randomly evolving enzymatic activities (promiscuity) are a raw material for fast adaptation. In our laboratory we are exploring to what extent are promiscuous enzymes mediating biofilm adaptation to novel environments and stressful conditions.

Engineering biofilms for plastic decontamination

Combining semi-rational protein and metabolic engineering we are developing synthetic biofilms for the depolymerization and assimilation of recalcitrant plastic polymers.